study of carbon nanotubes effects on the chondrogenesis of human adipose derived stem cells in alginate scaffold
نویسندگان
چکیده
background: osteoarthritis is one of the most common diseases in middle‑aged populations in the world and could become the fourth principal cause of disability by the year 2020. one of the critical properties for cartilage tissue engineering (te) is the ability of scaffolds to closely mimic the extracellular matrix and bond to the host tissue. therefore, te has been presented as a technique to introduce the best combination of cells and biomaterial scaffold and to stimulate growth factors to produce a cartilage tissue resembling natural articular cartilage. the aim of study is to improve differentiation of adipose derived stem cells (adscs) into chondrocytes in order to provide a safe and modern treatment for patients suffering from cartilage damages. methods: after functionalization, dispersions and sterilizing carbon nano‑tubes (cnts), a new type of nanocomposite gel was prepared from water‑soluble cnts and alginate. adscs seeded in 1.5% alginate scaffold and cultured in chondrogenic media with and without transforming growth factor‑β1 (tgf‑β1) for 7 and 14 days. the genes expression of sex determining region y‑box 9 (sox9), types ii and x collagens was assessed by real‑time polymerase chain reaction and the amount of aggrecan (agc) and type i collagen was measured by elisa. results: our findings showed that the expression of essential cartilage markers, sox9, type ii collagen and agc, in differentiated adscs at the concentration of 1 μg/ml cnts in the presence of tgf‑β1 were significantly increased in comparison with the control group ( p < 0.001). meanwhile, type x collagen expression and also type i collagen production were significantly decreased ( p < 0.001). conclusions: the results showed that utilized three‑dimensional scaffold had a brilliant effect in promoting gene expression of chondrogenesis.
منابع مشابه
Designing of Human Cartilage Tissue, by Differentiation of Adipose-Derived Stem Cells With BMP-6 in Alginate Scaffold
Purpose: In the present study the effect of BMP-6 was investigated on chondrogenesis of adiposederived stem cells. Materials and Methods: Mesenchymal stem cells derived from subcutaneous adipose tissue were cultured on alginate scaffold to induce chondrogenesis in experimental group, with chondrogenic medium having BMP-6 growth factor for 3 weeks. In control group medium without BMP-6 was appli...
متن کاملThe effect of cerebrospinal fluid-derived exosomes on neural differentiation of adipose mesenchymal stem cells in alginate hydrogel scaffold
Nowadays, researchers have made extensive efforts to find new treatments for nerve damage. Meanwhile, the role of exosomes in cell-cell communication is considered to be a new mechanism. Exosomes can act as suitable differentiating agents. The aim of this study was to investigate the differentiating effect of cerebrospinal fluid-derived exosomes on adipose mesenchymal stem cells in alginate hyd...
متن کاملThe effect of high frequency electric field on enhancement of chondrogenesis in human adipose-derived stem cells
Objective(s):Osteoarthritis (OA) is globally one of the most common diseases from the middle age onwards. Cartilage is an avascular tissue therefore it cannot be repaired in the body. Conservative treatments have failed as a good remedy and cell therapy as a decisive cure is needed. One of the best and easily accessible cell sources for this purpose is adipose-derived stem cells which can be di...
متن کاملImproving the Growth Rate of Human Adipose-Derived Mesenchymal Stem Cells in Alginate/Gelatin Versus Alginate Hydrogels
Background: Expansion and differentiation of stem cells relies on the soluble materials as well as the physical conditions of their microenvironment. Several methods have been studied in attempt to enhance the growth and differentiation rates of different adult stem cells extracted from different sources. Objectives: The purpose was to improve the three-dimensional (3D) culture condition of th...
متن کاملCytotoxicity of Chitosan Derived from Shrimp for Bone Scaffold on Adipose Tissue-Derived Mesenchymal Stem Cells
متن کامل
Effects of gamma radiation on adipose-derived mesenchymal stem cells of human breast tissue
Background: During radiation therapy, stromal cells surrounding the tumor (e.g mesenchymal stem cells) may affect the treatment outcomes. We aimed to investigate the effects of gamma radiation on the mRNA expression of cytokines, DNA damage and population doubling time (PDT) of adipose-derived mesenchymal stem cells (ASCs). Material and methods: ASCs were enzymatically extracted from breast tis...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
international journal of preventive medicineجلد ۵، شماره ۷، صفحات ۸۲۵-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023